By Feng Zhang, Holger Puchta, James G. Thomson
Over the earlier 50 years, biotechnology has been the main motive force for expanding crop productiveness. rather, advances in plant genetic engineering applied sciences have spread out massive new possibilities for plant researchers and breeders to create new crop types with fascinating qualities. fresh improvement of special genome amendment tools, corresponding to specific gene knock-out/knock-in and detailed gene alternative, strikes genetic engineering to a different point and provides much more potentials for making improvements to crop creation. The paintings offers an summary of the newest advances on special genomic engineering applied sciences in vegetation. subject matters contain recombinase and engineered nucleases-mediated specified amendment, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. ultimately, demanding situations and affects of the hot applied sciences on current laws for genetic amendment organisms (GMOs) might be discussed.
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Additional resources for Advances in New Technology for Targeted Modification of Plant Genomes
2013) Marton et al. (2010) Petolino et al. (2010) Townsend et al. (2009) Cai et al. (2009) 44 Y. Qi Modifying the plant genomes with ZFNs is not a trivial process. It will be helpful if activities and/or toxicities of ZFNs can be tested and measured by an easy reporter system. Multiple such systems have been developed. For example, a bacterial twohybrid reporter assay was successfully implemented for rapid assessment of ZF DNA-binding affinity in E. coli (Wright et al. 2006). A yeast-based assay was used for testing cleavage activities of ZFNs in vivo, which predicted in planta activities very well when ZFNs were expressed in Arabidopsis (Zhang et al.
Mol Ther 19(4):694–702 Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6):468–476 2 Engineering Meganuclease for Precise Plant Genome Modification 37 Hu D, Crist M, Duan X, Gimble FS (1999) Mapping of a DNA binding region of the PI-SceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis. Biochemistry 38(39):12621–12628 Ichiyanagi K, Ishino Y, Ariyoshi M, Komori K, Morikawa K (2000) Crystal structure of an archaeal intein-encoded homing endonuclease PI-PfuI.
2012). Many web-based tools are available for designing and selecting ZFNs. Zinc Finger Tools allows users to search for ZFNs which can be made by modular assembly (Mandell et al. 2006). ZiFit can identify potential ZFN sites in a given DNA sequence to facilitate engineering of ZFNs by the OPEN or CoDA method (Sander et al. 2010b). 0 version has included all possible ZFAs that can be generated by the CoDA method (Fu et al. 2009; Fu and Voytas 2013). ZFNGenome is a genome browser-based tool which helps locate potential ZFN target sites in multiple model organisms including Arabidopsis and soybean (Reyon et al.